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KMID : 1094720080130030313
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 3 p.313 ~ p.318
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Comparison of P aprE , P amyE , and P P43 promoter strength for β-galactosidase and staphylokinase expression in Bacillus subtilis
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Kim June-Hyung
Kim Byung-Gee
Hwang Bum-Yeol
Roh Ji-Won
Lee Jong-Ki
Kim Kwang
Wong Sui-Lam
Yun Hyung-Don
Lee Sun-Gu
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Abstract
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Selecting an appropriate promoter based on its expression ability is one of the most important points to consider when producing foreign proteins in a bacterial host. In this study, we compared the strength of three promoters (P aprE , P amyE , and P P43 ), all of which are widely used for foreign protein production in Bacillus subtilis. P aprE , P amyE , and P P43 promote the transcription of serine alkaline protease, 1,4-alpha-D-glucan glucanohydrolase, and cytidine/deoxycytidine deaminase, respectively. Among the single-copy chromosome-integrated forms of the β-galactosidase expression systems, P p43 demonstrated the highest expression level (200 Miller units of β-galactosidase expression), while P amyE and P aprE exhibited 70 and 10% of P P43 -driven expression, respectively. During multi-copy (50 copies per cell) plasmid-based expression of a therapeutic model protein, staphylokinase, the order of relative strength among the three promoters was similar to that observed in the single-copy integrated system; P P43 -driven expression yielded 120 mg/L staphylokinase.
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KEYWORD
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promoter, expression system, Bacillus subtilis, β-galactosidase, staphylokinase
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